Isolation, Purification and Characterization of Secondary Structure and Kinetic Study of Lipase from Indian Major Carp, Catla catla (Catla)

Lipases (E.C. 3.1.1.3) can be broadly defined as enzymes that catalyze the hydrolysis of ester bonds in substrates such as vitamin esters, phospholipids, triglycerides (TGs) and cholesteryl esters [1]. Interest in lipases has been increasing in the past few years because of the actual and potential uses of this enzyme in applications like hydrolysis of fats and oils, organic synthesis, modification fats, chemical analysis and flavor enhancement in food production. The isolation and purification of lipases is reported in various models, chiefly microorganisms but there is a paucity of information about the isolation and purification from carps. Therefore, the lipases from the tissues of these aquatic vertebrates have received much less attention than those from microorganisms. Hepatopancreas as digestive organs found in the fishes remain unexplored lipolytic enzyme sources that could have unique characteristics. Fish lipases demonstrate novel activities that have potential industrial applications [2]. Purification of lipase from the Indian Major Carps may also be of benefit for the development in the field of aquaculture. Thus the present study intricacies on the isolation purification and characterization of lipase from the digestive gut of Indian Major carp, Catla catla (catla). Materials and Methods